| Congresso Brasileiro de Microbiologia 2023 | Resumo: 339-2 | ||||
Resumo:Candida albicans is of great relevance in the healthcare field, as it has the potential to cause serious infections in patients with debilitating or immunosuppressed clinical conditions. This leaven is clinically known to be commensal of the mucous membranes in humans. However, it has pathogenic potential and is the cause of superficial and systemic candidiasis. The list of commercially available antifungals used for the treatment of C. albicans infections is extensive, with indications that vary depending on the patient's clinical status. Those who stand out are Miconazole, ketoconazole, clotrimazole, itraconazole and fluconazole, that belong to the class of azoles, in addition to amphotericin B and nystatin, of the class of polyenes. However, C. albicans can show resistance and patients may develop adverse effects to these drugs, resulting in the search for alternative treatments. The goal of this study was to evaluate the in vitro efficacy of fluconazole associated with ultrasound against C. albicans isolates. The standard strain of C. albicans (ATCC10231) was previously selected. The cultivation was carried out using the technique of seed depletion in Sabouraud Dextrose Agar (SDA) medium and incubated for 24 hours at 37°C. Then, fungal suspensions were prepared at a concentration of 1.5 x 108 CFU/ml, using the Mc Farland scale n° 0.5. 1ml of the suspension was transferred to Petri dishes containing 8ml of Sabouraud Dextrose Broth. A container was made and adapted from a 5-liter plastic gallon in order to allow the use of the ultrasound transducer submerged in water on the Petri dishes. The plates were divided into two groups: The control group (CG), where there was no addition of fluconazole and ultrasound treatment, and the experimental group (EG) which had the addition of fluconazole (FLU) (0.0012g/100ml broth) associated with ultrasound (UST) at 1 W/cm2 and a frequency of 1MHz. The GC and GE suspensions were inoculated in plates with SDA and incubated for 24 hours at 37º C. Ultimately, the C. albicans Colony Former Unity (CFU) growth was compared using the Promega Colony Counters program. For the association of UST + FLU, a count of 534 CFU was obtained, with an area of 7.8%; for the CG, 948 CFU were obtained, with an area of 47.2%. Thus, the antifungal in association with ultrasound seems to demonstrate efficacy. As this is a pilot project, more detailed analyzes are needed to suggest the studied protocol as a future alternative in the treatment of C. albicans in the health network.
Palavras-chave: C. albicans, ultrasound, fluconazol Agência de fomento:capes | |||||